Composite

Part:BBa_M36725

Designed by: Tammy Hsu   Group: Stanford BIOE44 - S11   (2011-04-28)


short-chain alkane hydroxylating device (mmoXYBZDC)

The mmoXYBZDC gene cluster is a methane monooxygenase found in methylococcus capsulatus. It catalyzes a C-H bond in methane, converting it to methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 326
    Illegal PstI site found at 692
    Illegal PstI site found at 2048
    Illegal PstI site found at 3794
    Illegal PstI site found at 4336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 326
    Illegal PstI site found at 692
    Illegal PstI site found at 2048
    Illegal PstI site found at 3794
    Illegal PstI site found at 4336
    Illegal NotI site found at 1911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1236
    Illegal BamHI site found at 1270
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 326
    Illegal PstI site found at 692
    Illegal PstI site found at 2048
    Illegal PstI site found at 3794
    Illegal PstI site found at 4336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 326
    Illegal PstI site found at 692
    Illegal PstI site found at 2048
    Illegal PstI site found at 3794
    Illegal PstI site found at 4336
    Illegal NgoMIV site found at 1868
    Illegal NgoMIV site found at 2704
    Illegal NgoMIV site found at 2888
    Illegal NgoMIV site found at 4633
    Illegal NgoMIV site found at 4811
    Illegal AgeI site found at 2115
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4772
    Illegal BsaI site found at 4837
    Illegal BsaI.rc site found at 245
    Illegal BsaI.rc site found at 383
    Illegal BsaI.rc site found at 800
    Illegal BsaI.rc site found at 1934
    Illegal BsaI.rc site found at 3938
    Illegal BsaI.rc site found at 4880


[edit]
Categories
Parameters
None